Rapid Affinity-Based Fingerprinting of 14-3-3 Isoforms Using a Combinatorial Peptide Microarray
نویسندگان
چکیده
منابع مشابه
Identification of 14-3-3 Proteins Phosphopeptide-Binding Specificity Using an Affinity-Based Computational Approach
The 14-3-3 proteins are a highly conserved family of homodimeric and heterodimeric molecules, expressed in all eukaryotic cells. In human cells, this family consists of seven distinct but highly homologous 14-3-3 isoforms. 14-3-3σ is the only isoform directly linked to cancer in epithelial cells, which is regulated by major tumor suppressor genes. For each 14-3-3 isoform, we have 1,000 peptide ...
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We devised a strategy of 14-3-3 affinity capture and release, isotope differential (d(0)/d(4)) dimethyl labeling of tryptic digests, and phosphopeptide characterization to identify novel targets of insulin/IGF1/phosphatidylinositol 3-kinase signaling. Notably four known insulin-regulated proteins (PFK-2, PRAS40, AS160, and MYO1C) had high d(0)/d(4) values meaning that they were more highly repr...
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The 14-3-3 proteins are acidic, dimeric proteins that have been implicated in many eukaryotic cellular processes because of direct protein association with enzymes and other metabolic and regulatory proteins. 14-3-3 proteins are largely considered to be cytoplasmic, but a search for proteins that specifically interact with a plant 14-3-3 resulted in the isolation of a nuclear-encoded, thylakoid...
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p53 maintains genome integrity by initiating the transcription of genes involved in cell-cycle arrest, senescence, apoptosis and DNA repair. The activity of p53 is regulated by both post-translational modifications and protein-protein interactions. p53 that has been phosphorylated at S366, S378 and T387 binds 14-3-3 proteins in vitro. Here, we show that these sites are potential 14-3-3 binding ...
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Cholangiocarcinoma (CCA) is an adenocarcinoma arising from the epithelial tissue of the intra-hepatic (10%), hepatic hilar (25%) or extrahepatic (65%) bile ducts (Lim, 2003). It is accounting for approximately 3% of all gastrointestinal malignancies (Wijaya and Abdullah, 2011). Recent data show that the incidence of cholangiocarcinoma have been increasing in several areas worldwide (Shu et al.,...
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ژورنال
عنوان ژورنال: Angewandte Chemie
سال: 2008
ISSN: 0044-8249,1521-3757
DOI: 10.1002/ange.200801395